Description

This service includes immunostaining, mounting and/or coverslipping as well as labeling the slides. As a result, you will receive colorimetric immunostained tissue sections ready for microscopic observations.

SI107-1    Colorimetric immunostaining with 1 primary antibody on free-floating sections, cf. photo samples

SI107-2   Colorimetric immunostaining with 1 primary antibody on sections mounted on slides, cf. photo samples

SI107-3   Colorimetric immunostaining with 2 primary antibodies on free-floating sections, cf. photo samples

SI107-4   Colorimetric immunostaining with 2 primary antibodies on sections mounted on slides.

Procedure:  Sections will be processed free-floating or on slides for immunostaining with 1 or more specific antibodies according to the avidin-biotin-complex (ABC) method¹.

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide fixed (or unfixed frozen) tissue sections and the specific antibodies.
• Please contact us for more information.

Reference:

1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.