Description

This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.

Procedure:  Following cryopretection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated slides (cf. Products, Cat. #PO101). Sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with 1 specific antibody according to the avidin-biotin-complex (ABC) method¹ (cf. photo samples below).

	Cytokeratin 18-immunostained section counterstained with cresyl violet. This 12 µm frozen section of the rat prostate was processed for Cytokeratin 18-immunoreactivity (brown) and was then counterstained with FD cresyl violet solution (cf. Products, Cat. #PS102) (click to see enlarged photo)
	CD31-immunostained section counterstained with hematoxylin. This 7 µm paraffin section of the mouse ear was processed for CD31-immunoreactivity (brown) and was then counterstained with FD hematoxylin solution (cf. Products, Cat. #PS104) (click to see enlarged photo).

Remarks:

• A quotation is required before placing an order.
• The investigator needs to provide fixed (or unfixed frozen) tissue and the specific antibody.
• Please contact us for more information.

Reference:

1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.