Several basophilic dyes can be used for Nissl staining. Thionin and azure II impart a deep blue color to Nissl substance of neurons and a pale blue color to the nuclear chromatin of both neuronal and non-neuronal cells. A similar staining pattern is also obtained with cresyl violet, which yields a violet color (cf. photo samples below).
|Nissl stain. 20 µm cryostat section cut transversely from the rat spinal cord was stained with FD crysel violet.|
|Nissl stain. High magnification of the ventral horn from the same section as shown above.|
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