The β-galactosidase gene is often used as a reporter gene, “marker” that is widely used for analysis of mutationally altered genes, as well as gene regulation. The expression of β-galactosidase gene can be detected by histochemical staining of tissue sections or cells. The method is based upon the ability of β-galactosidase to hydrolyze 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal), resulting in a blue staining in the cells expressing β-galactosidase¹.
This service includes tissue preparation, sectioning, histochemical staining, counterstaining (optional), and coverslipping. As a result, your will receive X-Gal stained sections ready for microscopic observations.
- A quotation is required before placing an order.
- The investigator needs to provide unfixed and freshly frozen tissue.
- Please contact us for more information.
- Horvitz, J P et al. (1964) J. Med. Chem. 7:574.